Biochemical identification was carried out by adding 0.6 ml of Barritt Reagent 'A' (1735A) and 0.2 ml of Barritt Reagent 'B' (1735B) in 24-48 hours old cultures grown in MR-VP Medium
Enterobacter aerogenes ATCC 1304
Growth - Luxuriant, VP Test-Positive (Cherry red color formation)
Escherichia coli ATCC 25922
Growth - Luxuriant, VP Test-Negative (No red color formation)
Klebsiella pneumoniae ATCC 13883
Growth - Luxuriant, VP Test-Positive (Cherry red color formation)
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Note : On storage of the reagent, precipitate may develop.This will not affect the performance criteria of the reagent.